ArgPure Nickel sepharose 6B Fast Flow
(Ni-S54)
for
Histidine-Tagged Protein Purification
Sepharose is a tradename for a crosslinked, beaded-form of agarose , a polysaccharide polymer material extracted from seaweed. A common application for the material is in chromatographic separations of biomolecule.
Purifying histidine-tagged proteins by immobilized metal affinity chromatography (IMAC) is a good choice for purification at a variety of scales. Also Nickel (Ni2+) is the most commonly used metal ion in IMAC purifications.
ArgPure Nickel Sepharose 6B Fast Flow (Nickel Agarose) is a high binding-capacity IMAC chromatography resin for purifying his-tagged proteins. Specifications of manufactured Nickel Sepharose by Noavaran Zist Gostar ARG Company is as follows:
| 2 | 1 |
| Affinity chromatography, Hepharin, IMAC Matrices, Molecular biology chromatography, Protein chromatography, Proteomics | Related categories |
| pack of 25 ml | Packaging |
| Ni-S54 | Mfr. no |
| 6% cross-linked agarose | Matrix |
| 40-180 μm | Particle size |
| ~ 90 μm | Average diameter |
| 2-14 (Ni2+ -stripped medium.) | Cleaning in place |
| 3-12 (Ni2+ -stripped medium.) | Working range |
| 29.5 mg Ni loaded on 1 g sepharose | Capacity |
| Suitable for bioprocess medium | suitability |