Nickel-NTA Sepharose Fast Flow Manufacturing by ARG Biotech Co
Sepharose is a tradename for a crosslinked, beaded-form of agarose , a polysaccharide polymer material extracted from seaweed. A common application for the material is in chromatographic separations of biomolecules.
Purifying histidine-tagged proteins by immobilized metal affinity chromatography (IMAC) is a good choice for purification at a variety of scales. Also Nickel (Ni2+) is the most commonly used metal ion in IMAC purifications.
Nickel-NTA Sepharose Fast Flow Resin (Nickel-NTA Agarose) is a high binding-capacity IMAC chromatography resin for purifying his-tagged proteins. Specifications of manufactured ArgPure Ni-NTA Sepharose Fast Flow Resin (Ni-NTA Agarose) by Noavaran Zist Gostar ARG Company is as follows.
Characteristics of ArgPure Ni-NTA Sepharose Fast Flow
Grade | BioProcess resin |
| Matrix | 6% cross-linked agarose |
| Ligand | Nickel-Nitrilotriacetic acid |
| Average particle size | 40-175 μm |
| Average diameter | ~ 100 μm |
| pH stability | 2–14 |
Chemical stability | Aqueous buffers: 0.1 M HCl, 0.1 M NaOH, 8 M Urea |
| Binding Capacity/ml Resin | Approx. 50 mg histidine–tagged protein |
| Pressure/Flow Specification | 250-400 cm/h, 100 kPa, XK 50/60 column, bed height 25 cm |
Certificate of Analysis ArgPure Ni-NTA Sepharose Fast Flow for His-Tagged Proteins
| Product No | Ni.S60 |
| Lot No: | A2022TB2 |
| Pack size | 25 ml |
| Type | Affinity chromatography, IMAC Matrices, Molecular biology chromatography, Protein chromatography |
| Storage temperature | 4 to 20°C |
| Storage buffer | 20% Ethanol |
As one of the companies established in East Azarbaijan Science and Technology Park, Noavaran Zist Gostar ARG company (ARG Biotech) is the first and only manufacturer of protein, antibody, enzyme, peptide and DNA purification resins in the Iran and Middle East.
Products manufactured by Arg Biotech Co in different grades are similar to samples supplied by reputable international companies and the high technical knowledge used in their design and manufacture process enhances the speed, accuracy and optimum purification efficiency of various macromolecules by biotechnology researchers.
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