Ni-NTA Sepharose Fast Flow

Ni-NTA Sepharose Fast Flow

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ArgPure Ni-NTA Sepharose Fast Flow



Histidine-Tagged Protein Purification

Sepharose is a tradename for a crosslinked, beaded-form of  agarose , a polysaccharide polymer material extracted from seaweed. A common application for the material is in chromatographic separations of biomolecule.

Purifying histidine-tagged proteins by immobilized metal affinity chromatography (IMAC) is a good choice for purification at a variety of scales. Also Nickel (Ni2+) is the most commonly used metal ion in IMAC purifications.

ArgPure Ni-NTA Sepharose Fast Flow is a high binding-capacity IMAC chromatography resin for purifying his-tagged proteins. Specifications of manufactured Nickel Sepharose by Noavaran Zist Gostar ARG Company is as follows:

Affinity chromatography, Hepharin, IMAC Matrices, Molecular biology chromatography, Protein chromatography, ProteomicsRelated categories
pack of 25 mlPackaging
Ni-S60Mfr. no
6% cross-linked agaroseMatrix
40-180 μmParticle size
~ 90 μmAverage diameter
2-14 (Ni2+ -stripped medium.)Cleaning in place
3-12 (Ni2+ -stripped medium.)Working range
29.5 mg Ni loaded on 1 g sepharoseCapacity
Suitable for bioprocess mediumsuitability