ArgPure Ni-NTA Sepharose Fast Flow
Histidine-Tagged Protein Purification
Sepharose is a tradename for a crosslinked, beaded-form of agarose , a polysaccharide polymer material extracted from seaweed. A common application for the material is in chromatographic separations of biomolecule.
Purifying histidine-tagged proteins by immobilized metal affinity chromatography (IMAC) is a good choice for purification at a variety of scales. Also Nickel (Ni2+) is the most commonly used metal ion in IMAC purifications.
ArgPure Ni-NTA Sepharose Fast Flow is a high binding-capacity IMAC chromatography resin for purifying his-tagged proteins. Specifications of manufactured Nickel Sepharose by Noavaran Zist Gostar ARG Company is as follows:
|Affinity chromatography, Hepharin, IMAC Matrices, Molecular biology chromatography, Protein chromatography, Proteomics||Related categories|
|pack of 25 ml||Packaging|
|6% cross-linked agarose||Matrix|
|40-180 μm||Particle size|
|~ 90 μm||Average diameter|
|2-14 (Ni2+ -stripped medium.)||Cleaning in place|
|3-12 (Ni2+ -stripped medium.)||Working range|
|29.5 mg Ni loaded on 1 g sepharose||Capacity|
|Suitable for bioprocess medium||suitability|